Petite H(1), Frei V, Huc A, Herbage D. Author information: (1)Coletica, Lyon, France. Cross-linking of collagen-based biomaterials increases their strength and. By DANIEL HERBAGE, JOSETTE BOUILLET and JEAN-CLAUDE Solubilization of collagen from bovine articular cartilage with pepsin. The formation of new bonds between the denatured collagen chains and the proteoglycans can explain the D. Herbage, A. Huc, D. Chabrand, M.C. Chapuy.

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Anne-Marie Freyria 1 AuthorId: The Perfect Match for Cardiac Repair? Cytological evaluation of capsular tissue surrounding TCPL implant in adult rats.

Use of diphenylphosphorylazide for cross-linking collagen-based biomaterials.

Gelatine-Based Biomaterials and Their Biocompatibility: RosenthalGabriele J Koehler Anticancer research In order to validate and quantitate the extent of reaction, the degree of cross-linking was determined by the measure of the free primary amino group content of the samples.

Topics Discussed in This Paper. Immunologically active biomaterials for cancer therapy. Collagen as matrix for neo-organ formation by gene-transfected fibroblasts. Howard The Journal of biological chemistry Recently, we described an efficient cross-linking process via the formation of acyl azide groups on methylated carboxyl groups of collagen using hydrazine and nitrous acid referred to here as the hydrazine method.

AliDavid J Mooney Current topics in microbiology and immunology Showing of 71 extracted citations. Metals Organ Ceramics Chitosan polylactic acid-polyglycolic acid copolymer.


Cross-linking of collagen-based biomaterials increases their strength and persistence in vivo. Cell proliferation was only noted in the 10 6 cells-seeded sponges 4-fold increase after 4 weeks of culture.

Development and characterisation of a three-dimensional in vitro hepatic fish model to investigate xenobiotic collwgen Matthew G Baron These in vitro results indicate that both collagen matrices can support the development of tissue engineered cartilage. Collagen-based biomaterials as 3D scaffold for cell cultures: Skip to search form Skip to main content.

Use of diphenylphosphorylazide for cross-linking collagen-based biomaterials.

Native and DPPA cross-linked collagen sponges seeded with fetal bovine epiphyseal chondrocytes used for cartilage tissue engineering. Allogeneic Mesenchymal Stem Cells and Biomaterials: Cross-linking of dermal sheep collagen using a water-soluble carbodiimide.

ChevallayDr D. Vacanti Journal of cellular biochemistry. Similar decreases in berbage primary amino group content and increases in thermal stability were obtained for collagen films treated with DPPA, glutaraldehyde, or hydrazine.

Wednesday, December 13, – 2: By clicking accept or continuing to use the site, you agree to the terms outlined in our Privacy PolicyTerms of Serviceand Dataset License. Mouse fibroblasts in long-term culture within collagen three-dimensional scaffolds: With the rapid development in tissue engineering, these different biomaterials have been used as three-dimensional scaffolds and cell transplant devices.

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Specific cartilage collagens types II and XI were deposited in the matrix throughout the culture and traces of type I collagen were noticed only in the culture medium after weeks and 4 weeks in the case of 10 6 and 10 7 cells-seeded sponges, respectively.

DPPA-treated pericardium had a resistance to collagenase digestion similar to that of glutaraldehyde- or hydrazine-treated pericardium. Glycosaminoglycans accumulated in hwrbage matrix, up to 1.

Collagen References

Daniel Herbage 1 AuthorId: Treatment of native bovine pericardium with 0. Ham A Benghuzzi Biomedical sciences instrumentation Collagen-based biomaterials in the form of sponges bovine type I collagen, both native and cross-linked by treatment with diphenylphosphorylazide, noted control and DPPA sponges respectively were tested as three-dimensional scaffolds to support chondrocyte proliferation with maintenance of the phenotype in order to form neocartilage.

From This Paper Figures, tables, and topics from this paper. In this report, we propose a simpler, faster way to prepare acyl azide groups and to cross-link collagen-based biomaterials, using diphenylphosphorylazide DPPA as a reagent.

BonassarCharles A.

References Publications referenced by this paper. BiomaterialsElsevier,22, pp. Showing of 84 references. Monday, October 29, – 9: