El cultivo de protoplastos en cítricos y su potencial para el mejoramiento genético. Article (PDF Available) · July with 1, Reads. Export this citation. Results 80 – 90 Download Citation on ResearchGate | On Jan 1, , R.R. Rodríguez and others published Aislamiento y cultivo de protoplastos en maracuyá. Universidad de León – Grado en Biotecnología – Tercero Biotecnología – Biotecnología vegetal – Tema 9. Cultivo de protoplastos e hibridación somática.

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Protoplasts were precipitated by centrifugation at rpm during five minutes.

Tema 9. Cultivo de protoplastos e hibridación somática

Nineteen somatic hybrid plants were obtained and confirmed by RAPD analysis. A revised medium for rapid growth bioassays with tobacco tissue culture.

Universidad Nacional Protoplatos La Molina. Production of somatic hybrids between frost – tolerant Solanum commersonii and Solanum tuberosum: Under these conditions, calli hybrids follow the developmental pathway of the morphogenic parents. Molecular characterization of inter- and intra-specific somatic hybrids of potato using randomly amplified polymorphic DNA RAPD markers. Root tips were stained by lacto-propionic orcein.

Cultivo y fusion de protoplastos

Resistance to Phytophthora infestans in Solanum tuberosum and wild Solanum species. Plant Cell Rep 3: Earn hundreds of dollars each month by selling your written material to your fellow students.

Your fellow students know exactly where the pitfalls lie and what the key elements will be to your success in cultivl module. To select hybrids, regeneration of in vitro plants was subjected to RAPD analysis.


Using chemical prootoplastos electrical procedures Jones, protoplasts from different donor plants can be fused together and somatic hybrids regenerated from the fusion products.

Regenerants from the fusion experiments were propagated in vitro using the Murashige and Skoog medium. When colonies had developed, alginate beads were despolimeryzed using 20 mM sodium citrate solution in sorbitol 0,3 M, pH 7,4 and gently shaking. Characterization of d hybrids of potato by use of RAPD markers and isozyme analysis. Many wild Solanum species are regarded as important sources for disease resistance and tolerance to many abiotic stresses Hawkes, but their use in potato breeding is limited due to poor crossability and sterility of interspecific hybrids.

No generic book summaries, but the specific content you need to ace your exams.

Interspecific somatic hybridization between Solanum tuberosum L. Origins of cultivated potatoes and species relationships. Protoplsatos somatic hybrids between wild potato Solanum acaule Bitt. Avoid resits and achieve higher grades with the best study guides, textbook notes, and class notes written by your fellow students. These results showed that all hybrids were aneuploids Fig. The other parent, the wild species S.

The wild species S. None of the plants had a similar banding pattern in relation to each one of the parents.

Cultivo y fusion de protoplastos [1990]

Thanks to the study guides written by fellow students specifically for your courses, you will never miss a trick when it comes to your exams. Somaclonal variation in potato. Molecular genetic characterization of plant somatic hybrids. Microcalli were transferred onto MS medium to induce shoot regeneration under indirect light conditions. This technique is a tool for somatic hybrid characterization Baird et al. After protoplasts fusion and during plant regeneration it frequently happens that genetic changes such as variations in chromosome number occur.


When colonies dd into microcalli, the alginate beads protkplastos depolimerized. Somatic hybridization in the genus Solanum: A rapid calli proliferation was observed after colonies developed. The enzyme treatment was carried out overnight with protoplastox agitation. The RAPD analysis was carried out for all regenerated plants derived from calli of the combination S. Production and molecular characterization of Citrus intergeneric somatic hybrids between red tangerine and citrange.

Nineteen plants were regenerated from calli Fig. Plant Cell Rep Everything you need to know about selling on Stuvia.

Tissue culture procedures to produce somatic hybrids and the application of molecular biological tools for their analysis protoplastoz well established. Identification of somatic hybrids is a requisite for the effective exploitation of the protoplasts fusion in potato improvement.